|
Course information
CHEM 443L / BIOL 443L - Biochemistry Laboratory is a one-credit
project-based laboratory
course, serving as an introduction to basic methods and techniques
employed in biochemical research. A range of potential projects
are considered, offering students opportunity to develop skills
in wet-lab methods and applied computation in biochemistry.
Students will learn and apply to a semester-long project appropriate
techniques from among heterologous protein overexpression, protein
chromatography, gel electrophoresis, quantification of proteins,
methods of polymerase chain reaction (PCR) - including primer design,
spectrophometric methods for enzyme assays and inhibition studies,
protein crystallization, database retreival, sequence
and structural homology searches, use of proteomics tools and
molecular graphics applications. The student projects can carried
out individually or in small groups, and each student will be expected
to keep a complete up-to-date laboratory notebook and be capable
of explaining their project orally any given week in a group-meeting
format. Students are expected in many cases to arrange to carry
out laboratory work outside of the scheduled weekly lab period.
A grade for the class will be based upon ability to conceive, plan,
and carry out a project, meeting expectations for reporting throughout
the semester, and a final, formal written project report. |
|